Aditya Ageng Sulistio


3D printing alginate-gelatine crosslinked hydrogel for muscle tissue engineering


Investigator: Aditya Ageng Sulistio (Master student) Aditya Ageng Sulistio


Supervisor: Thomas Distler, Prof. Dr.-Ing. habil. Aldo R. Boccaccini

Skeletal muscle may be injured due to sharp or blunt trauma, ischemia as well as by myotoxic agents. The function of skeletal muscle may also be deteriorated by myopathies, such as Duchenne’s muscular dystrophy (DMD) [1]. The use of muscle tissue engineering may overcome the detrimental effect of using autografts, such as donor site morbidity as well as inadequate innervation and perfusion of the transferred muscle [2]. The main objectives of this master thesis are to investigate the influence of scaffold geometry on the proliferation and differentiation of muscle cells. The scaffold will be created by 3D bioplotting technique and the scaffold viability will be assessed in vitro. In this project alginate dialdehyde/gelatine (ADA-Gel) hydrogel will be used as the bioink owing to its reproducible processing capability and biocompatibility [3].

[1] Riboldi, S. A.; et al., Biomaterials 2005, 26 (22), 4606–4615.

[2] Kwee, B. J.; Mooney, D. J. Current Opinion in Biotechnology. 2017, pp 16–22.

[3] Zehnder, T.; et al., Materials 2016, 9(11), 887.