Study of cell adhesion and migration of neoplastic cells on soft micropatterned surfaces


Investigator: Andreas Schwab (Master Student) Andreas Schwab


Supervisor: Dr. Aldo Leal-Egana, Prof. Dr.-Ing. habil. Aldo R. Boccaccini

In cancer disease, cell metastasis depends on the existence of specific geometric and mechanical cues, inducing cells to polarize and to acquire a pathogenic phenotype [1]. During this research project, the activity of neoplastic cells adhered to soft patterned polyacrylamide gels [2] having defined topological and mechanical features will be investigated. Assays will be done at single-cell scale and will be focused on cell adhesion and migration. The final aim of the investigation is the generation of a novel and miniaturized Cancer-on-a-Chip, with the purpose to study cell sensitivity to chemotherapeutic agents.

[1] A.D. Doyle, F.W. Wang, K. Matsumoto, K.M. Yamada, One-dimensional topography underlies three-dimensional fibrillar cell migration, The Journal of cell biology 184 (4) (2009) 481–490.

[2] T. Vignaud, H. Ennomani, M. Thery, Polyacrylamide hydrogel micropatterning, Methods in cell biology 120 (2014) 93–116.