Biofabrication using Methylcellulose Based Bioink

 

Investigator: Dalia Dranseikiene (Master student) Dalia Dranseikiene

 

Supervisor: Supachai Reakasame, Prof. Dr.-Ing. habil. Aldo R. Boccaccini


Biofabrication is a widely used tissue engineering technique which allows pre-defined deposition of cell containing hydrogels (bioinks). Combination of materials like oxidized alginate (ADA) and gelatin could be used for bioinks due to suitable properties for cell growth [1]. However, further developments of bioinks is required to print complex 3D structures. In this work, the use of methylcellulose to improve the printability of ADA-gelatin bioink will be evaluated to achieve robust 3D robust constructs for biofabrication [2] The scope of the project includes hydrogel characterization as well as cell viability studies of the created 3D structures.

[1] T. Zehnder, B. Sarker, A. R. Boccaccini and R. Detsch, Evaluation of an alginate–gelatine crosslinked hydrogel for bioplotting, Biofabrication 7 (2015) 025001.

[2] K. Schütz et al., Three‐dimensional plotting of a cell‐laden alginate/methylcellulose blend: towards biofabrication of tissue engineering constructs with clinically relevant dimensions, J Tissue Eng Regen Med. 11 (2017) 1574-1587.